Study of human mesenchymal-hematopoietic cell crosstalk to model chronic inflammatory settings
PEREZ G. 1, FLACHER V. 2, MUELLER C. 3
1 Université de Strasbourg, Strasbourg, France; 2 Université de Strasbourg, Strasbourg, France; 3 Université de Strasbourg, Strasbourg, France
An effective immune response taking place in lymphoid tissue relies on the interactions between lymphoid stromal cells and hematopoietic cells. In analogy, it is thought that stromal cells participate in the inflammatory process occurring in organs that can progress in the formation of tertiary lymphoid structures. Because so far mouse models inadequately recapitulate these processes, we aim to establish a cell culture system to study human mesenchymal stromal cells and their interaction with hematopoietic cells. To this end, we identified CD34+, CD73+ (NT5E), CD90+ (THY1) mesenchymal stem cells and CD34+, CD73+ (NT5E), CD90+ (THY1) pericytes in human adipose tissue. Following a similar procedure, lymphoid PDPN+ T-zone reticular cells (TRCs) were identified in human tonsils. The cells were isolated and sorted from these tissues and cultured in different media to determine the optimal conditions for cell growth. We will now study how stromal cells alter hematopoietic cell activation and, vice versa, how immune reactions affect stromal cells. We hope that these in vitro assays allow to shed light on the cellular and molecular events underlying the generation of inflammatory stromal cells which would be beneficial in the case of cancer but detrimental for autoimmune diseases.