CXCL-8: a Signature of Severe Helicobacter pylori Infection and a trigger of Region-Dependent Immune Response in the stomach
EL FILALY H. 1, DESTERKE C. 2, OUTLIOUA A. 1, BADRE W. 3, RABHI M. 4, KARKOURI M. 5, RIYAD M. 6, KHALIL A. 7, ARNOULT D. 8, AKARID K. 1
1 Biochemistry, Biotechnology and Immunophysiopathology Research Team, Health and Environment Laboratory, Ain Chock Faculty of Sciences, Hassan II University of Casablanca., Casablanca, Morocco; 2 INSERM UMRS-1311, Faculty of Medicine, University of Paris-Saclay, Villejuif, France., Villejuif, France; 3 Gastroenterology Department, CHU IbnRochd, Casablanca, Morocco., Casablanca, Morocco; 4 Diagnostic Center, H˘pital Militaire d'Instruction Mohammed V, Mohammed V University, Rabat, Morocco., Rabat, Morocco; 5 Laboratory of Pathological Anatomy, CHU Ibn Rochd/Faculty of Medicine and Pharmacy, UH2C, Casablanca, Morocco., Casablanca, Morocco; 6 Research Team on Immunopathology of Infectious and Systemic Diseases, Laboratory of Cellular and Molecular Pathology, Faculty of Medicine and Pharmacy, UH2C, Casablanca, Morocco., Casablanca, Morocco; 7 Research Center on Aging, Faculty of Medicine and Health Sciences, Department of Medicine, University of Sherbrooke, Sherbrooke, Quebec, Canada., Sherbrooke, Canada; 8 INSERM, UMR_S 1197, H˘pital Paul Brousse, Villejuif, France; UniversitÚ Paris-Saclay, Paris, France., Paris, France
Helicobacter pylori infection is involved in development of diverse gastro-pathologies going from a simple gastritis to severe stages such as gastric cancer. Using cytokines-chemokine levels (IL-17A, IL-1β, and CXCL-8) in H. pylori-infected patients, our aim was to determine their ability to be potential infection's signatures as well as their impact on the immune response in regards to the main gastric regions: corpus and antrum. Multivariate level analysis with machine learning model were carried out using cytokines/chemokine levels of Moroccan patients. For further insight, Geo dataset was used to run enrichment analysis following CXCL-8 upregulation. Accordingly, we showed that the combination of cytokines-chemokine levels allowed prediction of positive H. pylori density score with less than 5% of miss-classification error, with fundic CXCL-8 being the most important variable for this discrimination. In addition, CXCL-8 dependent expression profile was associated different response when it comes to each region of the stomach. To conclude, our results suggest that CXCL-8 appears to not only be a signature of advanced H. pylori infection, which is translated the progression of this disease towards corpus, but also an inducer of region-dependent immune response in response to H. pylori, highlighting the importance of discriminating between them in this infection-associated physiopathology.