Spatial profiling in lymphoid organs using CODEX technology
REICHELT R. 1, ROTTMANN S. 1, LUKACS-KORNEK V. 1
1 Institute of Molecular Medicine and Experimental Immunology (IMMEI), University Hospital Bonn, Bonn, Germany
Lymph nodes (LNs) as secondary lymphoid organs are crucial for the induction of adaptive immune responses. Different LN stromal cell populations provide structural support as well as exert immunoregulatory functions during an arising immune response. Upon infection, the massive influx of immune cells and proliferation of lymphocytes demand immense changes in the LN stromal cells network and their signaling. The basic changes and underlying cellular and molecular mechanisms have been subject of multiple studies, but the LN reorganization has not been imaged in a dynamic way within the intact cellular environment of the tissue before.
Thus, the aim was to visualize and investigate LN reorganization using CODEX (CO-Detection by indEXing) technology, a platform enabling highly multiplexed biomarker analysis of single cells within a tissue while preserving their spatial location. In addition to the observation of global changes in the stromal compartment within the intact cellular environment of the LN tissue, the variety of markers allows exploring cellular clusters as well as spatial and functional interactions between different cell types, showing contribution of specific cell populations in distinct immune responses. The kinetics of cellular and humoral immune responses are investigated with regard to cellular organization and stromal-immune cell interaction. This might provide novel insight into how stromal-immune cell crosstalk can be utilized for more efficient immune responses within secondary lymphoid organs.